Table 1.
Measurement of OM expansion.
| Area of OM (μm2) | ||||||
|---|---|---|---|---|---|---|
| L-formsa (ampicillin) | L-form-like cellsb (sucrose) | |||||
| Time (min) | Cell 1 | Cell 2 | Cell 3 | Cell 1 | Cell 2 | Cell 3 |
| 0 | 0.114 | 0.093 | 0.11 | 0.089 | 0.068 | 0.047 |
| 10 | 0.118 | 0.101 | 0.118 | 0.089 | 0.072 | 0.047 |
| 20 | 0.123 | 0.101 | 0.123 | 0.089 | 0.068 | 0.047 |
| 30 | 0.127 | 0.11 | 0.127 | 0.089 | 0.068 | 0.047 |
| 40 | 0.131 | 0.11 | 0.127 | 0.089 | 0.072 | 0.047 |
| 50 | 0.135 | 0.114 | 0.131 | 0.089 | 0.068 | 0.047 |
| 60 | 0.14 | 0.118 | 0.14 | 0.089 | 0.068 | 0.047 |
| 70 | 0.14 | 0.123 | 0.144 | 0.089 | 0.072 | 0.042 |
| 80 | 0.148 | 0.127 | 0.152 | 0.089 | 0.072 | 0.047 |
| 90 | 0.148 | 0.131 | 0.152 | 0.089 | 0.072 | 0.047 |
| Increase over 90 min (%) | 29.8 | 40.8 | 38.2 | 0 | 5.8 | 0 |
L-forms were selected from a time-lapse experiment with ampicillin treatment used for Video S4 (Supporting Information).
L-form-like cells were selected from a time-lapse experiment with sucrose treatment used for Video S2 (Supporting Information).