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rPLF-1 promotes muscle cell proliferation. (a) C2C12 myoblasts were treated with rPLF-1 (100 nM) for 24 hr for proliferation. (b) Representative immunoblots and quantitative data show the dose-dependent levels of p-Akt, p-mTOR, p-p38MAPK, and p-Erk1/2 proteins in the cells. Results are mean ± SE (n = 3–6). ∗p < 0.05 and ∗∗p < 0.01 by Student's t-test or one-way ANOVA followed by Tukey's post hoc tests.
