Figure 14.

Silencing of M6pr impaired cellular functions and growth signaling in C2C12 myoblasts induced by rPLF-1. (a, b) Cells transfected with siM6pr for 48 hr were subjected to the analyses of cell proliferation, migration, and invasion with rPLF-1 (100 nM) (migration/invasion calculation: ×200 magnification). (c) Cells transfected with siM6pr for 48 hr were cultured in the presence or absence of rPLF-1 for 45 min and then were subjected to a western blot assay. Results are mean ± SE (n = 3–6). ^∗p < 0.05 and ^∗∗p < 0.01 by Student's t-test or one-way ANOVA followed by Tukey's post hoc tests.