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. 2021 Nov 20;2021:9202990. doi: 10.1155/2021/9202990

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Copyright © 2021 Hiroki Goto et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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rPLF-1 stimulated bone marrow (BM) MuSC production and mobilization in response to CTX injury. (a, b) Representative dot plots and quantitative data for the numbers of CD34⁺/integrin-α7⁺ MuSCs in BM and peripheral blood (PB). (c) After the isolation of BM-derived integrin-α7⁺ stem cells with magnetic beads, the cells were cultured on cover glasses for 24 hr and then subjected to double immunofluorescence with goat pAb against integrin-α7 (red) and mouse mAb against Ki67 (green). Representative double fluorescence images and quantitative data show the numbers of proliferating cells (×200 magnification). Results are mean ± SE (n = 7–8). ^∗∗p < 0.01 vs. corresponding control groups by Student's t-test.