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. 2021 Dec 14;2021:5791181. doi: 10.1155/2021/5791181

Figure 2.

Figure 2

Transfection efficiency and the effect of hsa-let-7b on the proliferation of PDLSCs. (a) The expression of hsa-let-7b during the osteogenic differentiation of PDLSCs for 0, 3, and 7, days, respectively. ∗∗P < 0.01. (b) Transfection efficacy of hsa-let-7b was measured at day 3 by qRT-PCR. (c) CCK-8 analysis showed that there was no significant difference between mimic and mimic NC at 0, 1, 3, 5, and 7, respectively. P > 0.05. (d) CCK-8 analysis showed that there was no significant difference between inhibitor and inhibitor NC at 0, 1, 3, 5, and 7, respectively. P > 0.05. (e, f) Cell proliferation of PDLSCs was detected by EdU staining and assay. P > 0.05. EdU: 5-ethynyl-2-deoxyuridine; mimic: hsa-let-7b mimics; mimic NC: mimics control; inhibitor: hsa-let-7b inhibitor; inhibitor NC: inhibitor control.