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. 2021 Dec 14;2021:5791181. doi: 10.1155/2021/5791181

Figure 3.

Figure 3

Hsa-let-7b inhibited the osteogenic differentiation of PDLSCs. (a, b) Results of ALP staining and ALP activity assay after 7 days of osteogenesis (scale bar = 100 μm). ∗∗P < 0.01. (c, d) ARS staining and CPC assay at day 14 after osteogenic induction (scale bar = 100 μm). ∗∗P < 0.01. (e) The expression of osteogenesis-related proteins (ALP, RUNX2, and OSX) after osteogenic induction 3 days of transfected PDLSCs. GAPDH served as the reference protein. (f) Relative grayscale analyses of (e). ∗∗P < 0.01. (g) The expression of osteogenesis-related genes (ALP, RUNX2, and OSX) in group mimic NC, mimic, inhibitor NC, and inhibitor at day 3, respectively. ∗∗P < 0.01. (h, i) The immunofluorescence staining of RUNX2 and ALP in transfected PDLSCs and matched controls (scale bar = 50 μm).