Figure 5.

CTHRC1 is a direct target of hsa-let-7b. (a) The wild-type CTHRC1-3′-UTR (WT) and mutant CTHRC1-3′-UTR reporter plasmid (MUT) were constructed. (b) Luciferase reporter assays in 293 T illustrated that hsa-let-7b could bind with CTHRC1. (c–e) Western blot and qRT-PCR analysis were performed to detect the protein and mRNA levels of CTHRC1 in group mimic NC, mimic, inhibitor NC, and inhibitor at day 3, respectively. ^∗∗P < 0.01. (f) The immunofluorescence staining of CTHRC1 in transfected PDLSCs and matched controls (scale bar = 50 μm).