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. 2021 Dec 14;2021:5791181. doi: 10.1155/2021/5791181

Figure 6.

Figure 6

CTHRC1 enhanced the osteogenic differentiation capacity of PDLSCs. (a) The result of qRT-PCR showed that transfection effect of Si-2 was better. ∗∗P < 0.01. (b) QRT-PCR showed the expression of osteogenesis-related genes (ALP, RUNX2, and OSX) downregulated. ∗∗P < 0.01. (c) Western blot analysis of osteogenic markers in PDLSCs transfected with Si-CTHRC1. (d) Relative grayscale analyses of (c). ∗P < 0.05, ∗∗P < 0.01. (e, f) 7 days after mineralization, ALP staining and ALP activity analysis were performed (scale bar = 100 μm). ∗∗P < 0.01. (g, h) Results of ARS staining and CPC assay at day 14 (scale bar = 100 μm). ∗∗P < 0.01. (i, j) Immunofluorescence assay revealed that the protein expression of RUNX2 and ALP was downregulated in Si-CTHRC1 group as compared with controls (scale bar = 50 μm).