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The sequences of primer for qRT-PCR analysis.
| Target gene | Primes | Sequences (5′-3′) |
|---|---|---|
| ALP | Forward | GACCTCCTCGGAAGACACTC |
| Reverse | TGAAGGGCTTCTTGTCTGTG | |
|
| ||
| RUNX2 | Forward | TCTTAGAACAAATTCTGCCCTTT |
| Reverse | TGCTTTGGTCTTGAAATCACA | |
|
| ||
| OSX | Forward | CCTCCTCAGCTCACCTTCTC |
| Reverse | GTTGGGAGCCCAAATAGAAA | |
|
| ||
| CTHRC1 | Forward | TCATCGCACTTCTTCTGTGGA |
| Reverse | GCCAACCCAGATAGCAACATC | |
|
| ||
| GAPDH | Forward | GAAGGTGAAGGTCGGAGTC |
| Reverse | GAGATGGTGATGGGATTTC | |
