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Severe impairment in Srf−/− ES cells of IEG induction by LPA. Quantitative RT-PCR measuring expression of Egr-1 (A) and c-fos (B) in ES cell lines of the indicated Srf genotypes. mRNA induction factors relative to prestimulation levels are given for the 30-min time points subsequent to LPA addition. Standard deviations representing five independent experiments are provided (see Materials and Methods).
