Table 2.
Comparison of yield and quality of RNA isolated from rabbit cartilage using modified methodologies*
| RNA Quality Comparison | Method 1a† |
Method 1b |
Method 1c |
Method 2a |
Method 2b |
Method 2c |
|---|---|---|---|---|---|---|
| Method Details | Trizol/RNeasy Hybrid with Proteinase K and QIAshredder | Trizol/RNeasy Hybrid with Proteinase K |
Trizol/RNeasy Hybrid without Proteinase K |
Modified RNaqueous with Proteinase K and QIAshredder | Modified RNaqueous with Proteinase K | Modified RNaqueous without Proteinase K |
| Number of samples tested |
24 | 9 | 1 | 1 | 2 | 2 |
| UV absorbance 260/280 (average) |
1.9 | 1.8 | 2.9 | 2.2 | 0.0‡ | 5.2‡ |
| UV absorbance 260/230 (average) |
1.2 | 0.9 | 1.8 | 1.0 | 0.0‡ | 0.0‡ |
| Bioanalyzer Concentration (ng/μl) (average) |
58 | 38 | 8.1 | 0.8 | 0.0 | 0.1 |
| Bioanalyzer Total Yield (μg) (average) |
1.7 | 1.1 | 0.3 | 0.03 | 0.0 | 0.0 |
| Bioanalyzer RIN Score (average) |
7.1 | 5.2 | Not applicable | 2.2 | Not applicable | Not applicable |
| Representative Bioanalyzer Trace |
|
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*Normal rabbit articular cartilage (3 mg per sample) was used for RNA isolation in each method; †Method chosen for microarray analysis; ‡UV data available for only one sample.