Table 3.
Quantity and quality of RNA isolated from cartilage samples harvested at 8 weeks post microfracture*
| Project samples† | NanoDrop One | BioAnalyzer (Pico & Nano chip assay) | |||||
|---|---|---|---|---|---|---|---|
| Sample ID | Treatment‡ | 260/280 | RNA concentration (ng/µl) | RNA yield (µg) | RIN | Estimated RNA concentration (ng/µl) | RNA yield (µg) |
| 1 | Membrane | 2.0 | 55.3 | 1.7 | 7.0 | 52.8 | 1.6 |
| 2 | scSOX9-A76E | 1.9 | 47.6 | 1.4 | 7.1 | 33 | 1.0 |
| 3 | scSOX9-A76E | 1.7 | 63.8 | 1.9 | 6.5 | 35.2 | 1.1 |
| 4 | scSOX9 | 1.8 | 9.8 | 0.3 | 6.9 | 12.4 | 0.4 |
| 5 | Normal | 2.0 | 22.0 | 0.7 | 7.6 | 13.2 | 0.4 |
| 6 | scSOX9-A76E | 2.0 | 42.7 | 1.3 | 8.3 | 62.4 | 1.9 |
| 7 | Normal | 1.9 | 3.5 | 0.1 | 7.5 | 2.2 | 0.1 |
| 8 | Normal | 2.0 | 8.2 | 0.2 | 6.8 | 10.1 | 0.3 |
| 9 | scSOX9 | 2.0 | 66.4 | 2.0 | 7.2 | 57.6 | 1.7 |
| 10 | scSOX9 | 2.0 | 87.4 | 2.6 | 6.5 | 140.8 | 4.2 |
| 11 | Membrane | 2.0 | 45.7 | 1.4 | 6.7 | 59.2 | 1.8 |
| 12 | Membrane | 1.9 | 60.1 | 1.8 | 6.7 | 54.4 | 1.6 |
*Data presented in this table were obtained for RNA isolated with Method 1a (Figure 1 and Table 1).
†Wet weight of cartilage samples ranged between 1.9 - 2.9 mg.
‡Treatment: After cartilage defect was created, microfracture was performed and supplemented with collagen membrane (Membrane) alone, or with collagen membrane carrying super-positive changed charged SRY-type high-mobility group box 9 (scSOX9) or a mutant scSOX9-A76E. Normal: normal cartilage was taken from the counter lateral knee. RIN: RNA integrity number.