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. 2022 Jan 1;19(1):195–204. doi: 10.7150/ijms.66929

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Fgl2 is necessary for autophagy, contributing to CSCC cell proliferation. (A) Autophagosomes were detected by transmission electron microscopy, and the magnified view shows autophagosomes indicated by red arrows. (B) Confocal laser scanning microscopy was used to examine the effect of downregulating Fgl2 expression on autophagy in CSCC cells. (C) Western blot analysis of the autophagy-associated proteins LC3-I/II and P62. (D) CCK-8 assays showed the attenuated proliferative effect of overexpressing Fgl2 in the presence of the autophagic inhibitor CQ (**P<0.01).