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. 2022 Jan 1;19(1):195–204. doi: 10.7150/ijms.66929

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Fgl2 interacted with Tyrobp, contributing to CSCC proliferation. (A) The STRING database shows the protein-protein interaction (PPI) network of Fgl2. (B) Immunofluorescence for colocalization of Fgl2 and Tyrobp proteins in CSCC cells. (C) The interaction between Fgl2 and Tyrobp was analysed by Co-IP assays. Anti-Fgl2 antibody was used for IP, and the immunoprecipitates were examined by Western blots. (D) Tyrobp expression was analysed by Western blot analysis when Fgl2 was knocked down. (E and F) CCK-8 assays showed that downregulating Tyrobp expression attenuated the proliferation-promoting effect of overexpressing Fgl2 in A431 and SCL1 cells (***P<0.001).