Figure 6.

Tyrobp induced ERK-dependent autophagy, resulting in CSCC cell proliferation. (A) Western blot analysis of ERK activation and autophagic activity in CSCC cells with Tyrobp knockdown. (B) CSCC cells were incubated for 72 h in different concentrations of ravoxertinib and analysed by CCK-8 assays. (C) Western blot analysis of ERK activation and autophagic activity in CSCC cells overexpressing Tyrobp in the presence or absence of 2 μM ravoxertinib. (D) CCK-8 assays showed that the effect of proliferation induced by overexpressing Tyrobp was blocked in the presence of ravoxertinib.