Figure 4.

G6PD changes cell cycle dynamics and facilitates ccRCC cells growth. (A-D) Stably transfected ACHN-G6PD^OE, Caki-1- G6PD^si and relevant control cells were subjected to cell cycle distribution analysis by PI staining and flow cytometry assay. (E-F) Cell proliferation abilities of ACHN-G6PD^OE, Caki-1- G6PD^si and relevant control cell lines were assessed by MTS assay at different time points (1~5 day). The statistical data represented three independent experiments, each performed in triplicate. Error bars represent the means ± SD. ns, Not significant, *p <0.05, **p <0.01, ***p <0.001 vs. Control or Non-silencer group (unpaired Student's t-test for B-C, Mixed ANOVA for E-F).