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. 2022 Jan 1;18(1):112–123. doi: 10.7150/ijbs.63388

Figure 2.

Figure 2

Rab31 promotes the migration and invasion of cervical cancer cells. (A) Two shRNA lentiviruses targeting Rab31 were used to infect HeLa and SiHa cervical cancer cells, and Western blotting showed that Rab31 was efficiently knocked down. (B) A wound-healing assay was used to detect the capability of cancer cells to migrate at 0 h, 24 h and 48 h. The statistical graph on the right is a quantitative analysis of the wound-healing measured at different time points. (C) The effect of Rab31 knockdown on cell migration and invasion was analyzed by Transwell assay. The pictures below showed the statistical results. (D) The Rab31 expression plasmid was transfected into C33A cells, and Rab31 expression was detected by Western blotting. (E) A wound-healing assay was performed and detected at 0 h, 24 h and 48 h. (F) The effect of Rab31 overexpression on cell migration and invasion was explored using Transwell assay. *P <0.05, **P <0.01, ***P <0.001.