Figure 3.

Rab31 promotes the migration and invasion via EMT and cytoskeletal rearrangement. (A) The effect of Rab31 knockdown on the morphology of SiHa cells was shown. (B) Western blotting of the protein levels of epithelial marker E-cadherin, mesenchymal markers (N-cadherin, Vimentin), EMT transcription factors Snail and Slug as well as MMP2, MMP9 in Rab31-knockdown HeLa and SiHa cells were shown. (C) Cellular immunofluorescence assays were performed to detect the expression of E-cadherin and Vimentin in HeLa and SiHa cells. (D) Expressions of EMT-related proteins in Rab31-overexpressing C33A cells were measured. (E) The filamentous actin backbone of HeLa and SiHa cells was fluorescently stained with phalloidin, and the cytoskeletal structure was observed under a fluorescence microscope. The arrow in the picture above indicates F-actin and the arrow in the picture below indicates the pseudopod. *P <0.05, **P <0.01, ***P <0.001.