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. 2022 Jan 1;18(1):112–123. doi: 10.7150/ijbs.63388

Figure 4.

Figure 4

Rab31 inhibits MAPK6 degradation. (A) The interaction of endogenous Rab31 and MAPK6 in SiHa cells was detected using the Co-IP assay. (B) Transfection of plasmids expressing Rab31 (Flag tag) and MAPK6 (HA tag) into SiHa cells and the interaction between exogenous Rab31 and MAPK6 was detected. (C) Detection of MAPK6 protein expression after Rab31 knockdown. (D) Western blotting of MAPK6 cellular localization following Rab31 knockdown was shown. (E) Cells with Rab31 knockdown were incubated with 5 μg/mL of CHX and harvested at the indicated times. MAPK6 stability was monitored by the line chart, and the half-life of MAPK6 was evaluated on the right. (F) Cells with Rab31 overexpression were incubated with 5 μg/mL of CHX and harvested at the indicated times. MAPK6 stability and the half-life of MAPK6 were shown. (G-H) The mRNA level of MAPK6 was detected following Rab31 knockdown or Rab31 overexpression using qRT-PCR. *P <0.05, **P <0.01, ***P <0.001.