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. 2022 Jan 1;18(1):112–123. doi: 10.7150/ijbs.63388

Figure 5.

Figure 5

Rab31 promotes the invasion of cervical cancer cells partly via MAPK6. (A) Detection of MAPK6 protein in HeLa and SiHa cells after MAPK6 knockdown. (B) MAPK6 was knocked down in HeLa and SiHa cells, and CCK-8 assay was performed. (C) The migration and invasion of HeLa and SiHa cells were tested by Transwell assay following MAPK6 knockdown, and the pictures below show the statistical results. (D) The viability of Rab31-overexpressing cells following MAPK6 knockdown was analyzed by the CCK-8 assay. The significant difference of viability between Rab31-overexpressing cells with MAPK6 knockdown and Rab31-overexpressing cells was marked with #. (E) The migration and invasion of Rab31-overexpressing cells following MAPK6 knockdown were tested by Transwell assay. The pictures on the right show the statistical results. (F) The effect of MAPK6 overexpression on the metastatic capability of Rab31-knockdown HeLa and SiHa cells was detected using Transwell assay and the pictures below show the statistical results. (G) EMT-related proteins in Rab31-knockdown cells with MAPK6 overexpression were detected by Western blotting. *P <0.05, **P <0.01, ***P <0.001, #P <0.05, ##P <0.01, ###P <0.001.