MiR-206 was a suppressive factor, and the downregulation of its expression promoted iCCA deterioration. (A-B) Low expression of miR-206 was detected in iCCA by qPCR (n=42) and FISH, scale bars =100 µm and 50 µm. (C-D) The overall survival time (n=42, P=0.0008) and disease-free survival time (n=42, p=0.0014) of patients in the relatively low and high miR-206 expression groups were analyzed and compared. (E) Alpha-SMA and miR-206 staining results in iCCA tissues. Scale bar= 50 µm. (F) Relationships between miR-206 expression and E-cadherin, Vimentin, VEGF-alpha and alpha-SMA expression in iCCA tissues were analyzed (n=28). The data were shown as the mean ± SD, ** P <0.01, *** P <0.001.