Figure 8.
Exosome-delivered miR-206 eliminated the CCA-CAF mutually promoting environment and suppressed malignancy. (A) The expression level of miR-206 in -derived exosomes transfected with the miR-206-mimic was detected by qPCR. (B-E) Effects of miR-206-mimic/exo (50 μg/105 cells) on HUCCT1 cell proliferation and migration were detected. The CCK-8, colony formation, cell cycle progression and Transwell migration (scale bar=20 μm) assay results are presented. (F) Levels of LASP1, Nanog, and p-STAT3 in HUCCT1 cells after miR-206-mimic/exo treatment were assessed. (G-H) The expression of CAF markers (alpha-SMA and FAP) and Anxa2 in CAFs was evaluated. (I) HUCCT1 cells and CAFs were cocultured in 6-well plates, and both cell types were treated with gemcitabine or miR-206-mimic/exo. The cells were analyzed compared to those cultured alone. Cell colony number and fold change in the gemcitabine and miR-206-mimic/exo treatment groups were compared and are presented in the right panel. (J) The Bcl-2/Bax ratio and fold change in the Bcl-2/Bax ratio in the gemcitabine and miR-206-mimic/exo groups were also evaluated and analyzed. *P <0.05, ** P <0.01, *** P <0.001.