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. 2022 Jan 1;18(1):441–458. doi: 10.7150/ijbs.64187

Figure 3.

Figure 3

Minocycline inhibits AQP4 expression and lessens the cerebral edema after TBI. (A) Wet/dry weight ratios of Sham, TBI and TBI+Mino mice in the ipsilateral cortex at day 3 after TBI. n=5 per group. *P < 0.05, **P < 0.01 vs. the indicated group. (B-C) Western blot analysis and quantification of AQP4 in ipsilateral cortex 3 day after TBI. n=3 per group. *P < 0.05, ***P < 0.001 vs. the indicated group. (D) Representative immunofluorescence images depicting GFAP (red) with AQP4 (green) at 3 dpi. Scale bar = 10 µm, n=3 per group. Six images below are used to describe the specific locations of AQP4 on astrocytes. (E-G) The overlap of fluorescence intensity peaks along profiles to show the co-localization of GFAP and AQP4 in each group. E: Sham group. F: TBI group. G: TBI+Mino group.