TABLE 2.
Estimated molecular weight range (kilodaltons) of polypeptides found in wet (WB), dried at low temperature (DB), and lyophilized (LB) Spirulina biomass.
| >75 kDa |
37–75 kDa |
25–37 kDa |
20–25 kDa |
<20 kDa |
|||||||||||
| 4–5 | 5–6 | 6–7 | 4–5 | 5–6 | 6–7 | 4–5 | 5–6 | 6–7 | 4–5 | 5–6 | 6–7 | 4–5 | 5–6 | 6–7 | |
| Wet biomass | |||||||||||||||
| WB | 1 | – | – | 1 | 28 | 5 | 4 | 22 | 9 | 7 | 13 | 9 | 5 | 18 | 12 |
| WBA | 1 | – | – | 1 | – | – | – | – | – | – | – | – | – | – | 1 |
| WBF | 1 | – | – | 1 | 18 | 4 | 4 | 18 | 7 | 3 | 13 | 8 | 4 | 21 | 8 |
| WBAF | 1 | – | – | 1 | – | – | – | – | – | – | – | – | – | – | 1 |
| Dried biomass | |||||||||||||||
| DB | 1 | – | – | 1 | 5 | 2 | 3 | 8 | – | 2 | 3 | 3 | 8 | 16 | 12 |
| DBA | 1 | – | – | 1 | – | – | – | – | – | 1 | – | – | – | 1 | – |
| DBF | 1 | – | – | 1 | 3 | – | 5 | 1 | – | 1 | 1 | – | 2 | 4 | 10 |
| DBAF | 1 | – | – | 1 | – | – | 1 | – | – | 1 | – | – | – | 3 | 3 |
| Lyophilized biomass | |||||||||||||||
| LB | – | 2 | 6 | 1 | – | 3 | 2 | 2 | 3 | 1 | – | 2 | 2 | – | 2 |
| LBA | 2 | – | – | 1 | 2 | 2 | – | 1 | 2 | 2 | – | – | 1 | 1 | 1 |
| LBF | – | – | 3 | 4 | 17 | – | 4 | 12 | – | 6 | 3 | – | 2 | 4 | – |
| LBAF | – | – | 2 | 3 | – | – | 1 | – | – | – | – | – | – | 1 | 1 |
All biomasses were also treated with Alcalase® (A, 1% vol/wt), fermented with Lactiplantibacillus plantarum T0A10 (F, final cell density of circa 7 log10 cfu/g), or fermented and treated with Alcalase® (AF). Enzymatic and microbial treatments were carried out at 30°C for 24 h.