Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 21;13(1):22. doi: 10.1038/s41419-021-04462-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — A The upregulated circRNAs were screened by two online databases, GSE100186 comprised of four paired ccRCC tissues and normal tissues in the GEO database as well as the Cancer-Specific CircRNA database. B The cluster heatmap was conducted to present the top 100 upregulated circRNAs of this intersection in the GSE100186 dataset. C circPPP6R3 was produced by back-spliced of the exon 18, 19, and 20 of the PPP6R3 gene identified by sanger sequencing. D The PCR products of circPPP6R3 and its linear gene PPP6R3 mRNA from the cDNA and gDNA of ccRCC cells were tested by Northern Blotting, GAPDH was used as a control. E Actinomycin D was applied to detect the stability of circPPP6R3 and PPP6R3 mRNA. F The expression of circPPP6R3 and PPP6R3 mRNA were validated by qRT-PCR under the digestion of RNase R in ccRCC cells. G The expression of circPPP6R3 was determined by qRT-PCR in ccRCC cell lines and the renal tubular epithelial cell line, HK-2. H qRT-PCR analysis of circPPP6R3 in 96 paired of ccRCC tissues and adjacent normal tissues. I The Kaplan–Meier analysis indicated that the ccRCC patients with high expression of circPPP6R3 had unfavorable disease-free survival rates compared with low circPPP6R3 expression.