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. 2021 Dec 21;13(1):22. doi: 10.1038/s41419-021-04462-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A qRT-PCR detected the expression of circPPP6R3 and PPP6R3 mRNA in Caki-1 and ACHN cells after transfected with siRNAs targeting circPPP6R3. B–D MTS assay, EdU incorporation assay, and colony formation assay were employed to detect the proliferation of ccRCC cells. E, F Transwell assay, invasion assay, and wound-healing assay were performed to detect the migrative and invasive abilities of ccRCC cells.