Table 2.

Oligonucleotide primers used in this study.

Primer name	Primer Sequence
SeqpBADdkcAF	GTAACAAAGCGGGACCAAAGC
SeqpBADdkcAR	GGCGTTTCACTTCTGAGTTCG
Primers used for site-directed mutagenesis study of DkcA protein
E40AFW	GTTCTGCGCAACCGGGCTGGTGGTGAC
E40AREV	CCCGGTTGCGCAGAACAGAATCAAAAACAGAATGGCATAAAC
R133AFW	CCTGGCGGCCTTTGTGCCGATAGTCCGAACCTTC
R133AREV	CACAAAGGCCGCCAGGATAATGGTTTTCCCGC
R139AFW	GATAGTCGCAACCTTCGCGCCGTTTGTG
R139AREV	GAAGGTTGCGACTATCGGCACAAAGCGCG
Primers used to knock out arnT from wild type K. pneumoniae ST258 background
arnT_KO_forward	GGGGCGCTAAAGCGCGGCTGGCTTGAGCGCGATGAGGCCCGCGGCGCGCTGTATCTGTTACGAATAAGGGACAGTGAAGAAGGAACACC
arnT_KO_reverse	ATAGGTCGGCAGTTTGCCTTTGGCGATACTGAAGAACAGGAACGGCATCGCCACCCAACCCTGCAGGAATTCGATGTGTAGGCTG
Primers used for the verification of insertion of arnT knock out cassette consisting apramycin selection marker
P1F	AATATTGAATTCCCTGCTGGACTGCATCCATCG
P2R	CGCTGCGCTTTACATTTGGCAG
P3F	CCTGCCAAATGTAAAGCGCAGC
P4R	ATTAATAAGCTTCAGAATGACAATGCCCACGACGATC
Primers used for cloning arnT into expression vector
KparnT1	ATTATACATATGATGAAAAGCATTCGCTATGGCGTCTC
KparnT2	AATATTTCTAGATTATTGCGGCAGGTACTGAAGGAAAAC