Table 1.

Characteristic of the ten most abundant rRFs/miRNAs expressed in Rhodotorula frigidialcoholis.

rRF/miRNA ID	Size (nt)	Origin	Differential expression	Potential targets	Binding energy (kcal/mol)
rRF/miRNA1	30	rRNA	Not differentially expressed	ATPase GET3	−41.6
				Metallo-dependent phosphatase	−38.8
				Protein with homology to sperm nuclear basic protein PL-I isoform PLIb	−38.8
rRF/miRNA2	18	rRNA (28S)	Overexpressed at 0 °C	Glutathione S-transferase	−34.7
				Unknown protein	−34.4
				Unknown protein with acetyl-CoA carboxylase/biotin carboxylase 1 domains	−32.7
rRF/miRNA3	28	rRNA (28S)	Overexpressed at 0 °C	ATP-NAD kinase	−43.2
				Unknown protein with RNA polymerase II degradation factor 1 domain	−41.2
				Ribosome biogenesis protein ERB1	−40.9
rRF/miRNA4	29	rRNA	Overexpressed at 23 °C	ATPase GET3	−41.6
				Protein with homology to sperm nuclear basic protein PL-I isoform PLIb	−39.3
				Ribosome biogenesis protein ERB1	−36.9
rRF/miRNA5	18	rRNA (28S)	Overexpressed at 0 °C	COP9 signalosome complex subunit 7	−34.9
				Mannosyl-oligosaccharide alpha-1,2-mannosidase	−34.5
				RNA polymerase II degradation factor 1	−32.9
rRF/miRNA6	20	rRNA (28S)	Overexpressed at 0 °C	RNA pol II promoter Fmp27 protein domain	−35.9
				NAD + kinase	−35.8
				RNA polymerase II degradation factor 1	−35.3
rRF/miRNA7	22	rRNA	Overexpressed at 0 °C	Staphylococcal nuclease domain-containing protein 1	−33.6
				Unknown protein	−32.8
				Glycogen synthase	−32.7
rRF/miRNA8	22	Unknown protein	Overexpressed at 0 °C	programmed cell death 6-interacting protein	−47.0
				Kinase-like protein	−46.1
				Gutaminyl-tRNA synthetase	−45.6
rRF/miRNA9	29	rRNA (25S)	Overexpressed at 0 °C	Transcription factor IIIB 90 kDa subunit	−51.8
				Unknown protein	−50.9
				Glycosylphosphatidylinositol inositol-deacylase	−50.3
rRF/miRNA10	17	rRNA (28S)	Overexpressed at 0 °C	COP9 signalosome complex subunit 7	−30.5
				26S proteasome regulatory complex component	−30.2
				DHS-like NAD/FAD-binding domain-containing protein	−28.9

Summary of the transcription origin, and differential expression of R. frigidialcoholis miRNAs. Putative genomic origin of the rRFs/miRNAs were predicted with BLAST [38] and their putative mRNA gene targets were predicted using the RNAhybrid computational method [40].