Fig. 6. Preference of Gram-positive bacteria and aversion of Gram-negative bacteria to synthetic leaf structures derived from high cytokinin content/signaling genotypes.

Indicated bacteria (OD₆₀₀ = 0.01) were spray inoculated onto agar replicas of leaves of the indicated genotypes. Bacterial growth was quantified after 24 h. Preference or aversion to the leaf structure was assessed by quantifying the colony forming units (CFU) growing on the leaf structure, and dividing it by the CFU growing on an equal area in the surrounding structure-less agar. Box plots are shown with inner quartile ranges (box), outer quartile ranges (whiskers), median (line), depicting results from three independent experiments, all points shown. A B. megaterium 4C. Letters indicate significance in a one-way ANOVA with a Dunnett post hoc test, N = 9, p < 0.0001. B B. pumilus R2E. Letters indicate significance in a one-way ANOVA with a Tukey post hoc test, N = 7, p < 0.04. C B. subtilis SB491. Letters indicate significance in a one-way ANOVA with a Tukey post hoc test, N = 6, p < 0.0016. D R. pickettii R3C. Letters indicate significance in a one-way ANOVA with a Tukey post hoc test, N = 6, p < 0.029. E P.  aeruginosa  IN68. Letters indicate significance in an unpaired two-tailed t test, N = 7, p < 0.019.