Fig. 4. Dissimilatory sulfite reductase (DsrA) phylogenetic tree showing the diversity of MAGs involved in dissimilatory sulfur cycling using the dsr system.
The tree includes 226 DsrA proteins identified in the 402 MAGs obtained in this study. These sequences are highlighted in blue text and labeled according to the 30 groups described in Fig. 1 (U and C for uncultured and cultured, respectively). The tree also contains 337 reference DsrA sequences from a manually curated database that includes representatives from public databases (see methods). The phylogeny was inferred using IQ-TREE (v.1.6.11, model LG + R7) with the ultrafast bootstrapping option -bb 1000. Numbers shown in parentheses are the number of protein sequences in a collapsed group belonging to the 402 MAGs.