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. 2021 Jul 1;16(1):26–37. doi: 10.1038/s41396-021-01045-2

Fig. 5. Amplification-based quantification (qPCR) of genes and transcripts.

Fig. 5

A Boxplot showing quantified gene copy abundance g−1 soil (wet weight). Primers targeting the 16S rRNA (27F/518R), nirK (517F/1055R), nirS (cd3aF/R3cd) and nosZ clade I (nosZF/1622R) genes were used for amplification. B Quantified mRNA transcript abundance g−1 soil (wet weight) of nirK, nirS, and nosZ. Red = SoilA (pH 3.8); Blue = SoilN (pH (6.8).