Fig. 2.
Comparison of DNA copy numbers of AcBac-Syn and AcBac-egfp-ph in E. coli. The E. coli EPI300 containing AcBac-Syn or AcBac-egfp-ph was cultured overnight with or without the presence of CopyControl Induction Solution (CopyControl, Epicentre). Total DNAs of 3 mL each E. coli culture (OD600 value 1.8) were extracted and suspended in 100 μL water. Quantitative PCR was performed to detect the AcBac-egfp-ph or AcBac-Syn DNA copy numbers. The copy numbers were the average value from three independent infections. Error bars represent standard deviations. *P < 0.05, P > 0.05 means no significant difference.