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. 2021 Jul 5;36(6):1400–1410. doi: 10.1007/s12250-021-00414-7

Fig. 4.

Fig. 4

Effect of siRNA-mediated DSG2 downregulation on HAdV attachment. A The Western blot results of DSG2 expression in A549 cells at 24, 48, 72 h after transfection with siRNA. B GFP expression in A549 cells transfected with siRNA and infected with HAdV-55-GFP and HAdV-3/-5-GFP. D The immunofluorescence in A549 cells after infection with HAdV-B55 and HAdV-B11/-B14. Mouse polyclonal antibodies against HAdVs (prepared in our laboratory) were incubated with the cells, then the second antibodies goat Anti-Mouse IgG H&L (FITC) (ab6785) was applied. C and E Mean fluorescence intensity values. n = 10. Note that at 18 h post-infection, GFP and immunofluorescence levels were comparable for HAdV-55-GFP and HAdV-3/-5/-11/-14-GFP. DSG2-siRNA and Control siRNA transfected cells were infected with HAdVs at 1 MOI. ***: P < 0.001. Scale bar = 100 μm.