Fig. 4.

MTase domain of NS5 and CTD of p53 mediates the NS5-p53 interaction. (A and B) Schematic diagram of truncation mutants of ZIKV-NS5 (A), and p53 (B). C and D MTase domain of ZIKV-NS5 and the C-terminal domain (CTD) of p53 mediated their interaction. HEK293T cells transfected plasmids that expressing ZIKV-NS5, MTase, RdRp or vector (C) and cells co-transfected ZIKV NS5-expressing plasmid with plasmids expressing p53, TAD + DBD, DBD, DBD + CTD, CTD or vector (D), were lysed and immunoprecipitated. The protein interactions were detected by Western-blot.