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. 2021 Dec 21;12:7362. doi: 10.1038/s41467-021-27365-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Analysis of mRNA expression by qRT-PCR shows that Plin2 is the most abundant Plin family member in quiescent SVZ NSPCs (n = 3 samples, fold change versus Plin1 ± SEM, one-way ANOVA with Dunnett’s multiple comparisons correction, adj. p-values: Plin2 < 0.0001, Plin4 = 0.0045). b Staining against PLIN2 (white) show that quiescent SVZ NSPCs also accumulate LDs, some of which have a larger size. Representative images are maximum intensity projections. Results were similar among 30 images from 3 coverslips per condition. DAPI-positive nuclei (blue). c The total LD accumulation did not significantly differ between proliferating and quiescent NSPCs (n = 378 proliferating cells, n = 567 quiescent cells from 3 coverslips per condition, mean ± SEM, Mann–Whitney test, two-tailed, p = 0.7). d Measurements of the diameter of LDs in proliferating and quiescent NSPCs revealed a significant increase in the proportion of large LDs with quiescence. Shown are violin plots depicting all LD diameters in the two conditions (Mann–Whitney test, two-tailed, p < 0.0001). e Stable overexpression of the Plin2-Gfp construct in NSPCs leads to GFP-positive LDs, as shown with PLIN2 co-staining. Representative images are maximum intensity projections. DAPI-positive nuclei (blue). Results were similar among 3 coverslips. f Time-lapse analysis of PLIN2-GFP NSPCs during quiescence induction. Representative brightfield (upper panel) and fluorescent images (lower panel) at 0, 24, 48 and 72 h after quiescence induction. Results were similar among 9 large fields of view from 3 wells. g Quantification of LDs over time revealed a significant increase in the number of LDs in quiescent NSPCs. (n = 3 wells, mean ± SEM, one-way ANOVA, Tukey’s multiple comparisons test, adj. p-value: 0 h vs. 72 h = 0.0003). h LD diameters were also significantly increased when full quiescence was reached. Violin plots depict all LD diameters at the timepoints analysed (one-way ANOVA, Tukey’s multiple comparisons test, adj. p-values: 0 h vs. 48 h < 0.0001; 0 h vs. 72 h < 0.0001). Asterisks indicate the following p-values: *** < 0.001.