Fig. 5.

The role of TRIM27 in CVB3 replication. A, B HeLa cells were transfected with TRIM27 vector or si-TRIM27, and infected with CVB3 (MOI = 10) for 5 h and 8 h; total protein was extracted to analyze the expression level of VP1, TRIM27, ERK1/2 and p-ERK1/2, using GAPDH as a loading control. C Viral plaque assay. HeLa cells were transfected with miR-324-3p and miR-NC for 48 h, and infected with CVB3 (MOI = 0.01) for 72 h (n = 3). D HeLa cells were transfected with miRNAs and infected with CVB3 (MOI = 10) for 8 h. IF microscopy analyzed the expression of TRIM27. CVB3, coxsackievirus B3; MOI, multiplicity of infection, scale bar: 10 μm.