Fig. 7.
The role of miR-324-3p in VM. miR-324-3p was overexpressed or silenced by AAV injection in mice for 4 weeks. The mice were then infected with CVB3 (105 PFU/mouse) for next 3 weeks. Samples were collected for analysis. A RT-qPCR analysis was used to determine the expression of TRIM27 (n = 3). B Western blot analysis was used to determine the protein levels of VP1, TRIM27 and p-ERK1/2. GAPDH was used as a loading control. C The expression level of TRIM27 in mouse heart tissue was measured by immunohistochemical (IHC) analysis (original magnification × 400). D Heart tissue sections were prepared 3 weeks post-infection (n = 3). Histopathological evaluation was performed to detect the presence and severity of myocarditis in the heart tissues of different groups (original magnification × 400). The partial enlarged image enlarges 4.125 times of the original image, and the arrow points out where the infiltrating white blood cells. VM, viral myocarditis; AAV, adeno-associated viruses; CVB3, coxsackievirus B3.