TABLE 2.

The effect of genes selected to characterize the accumulation of isoflavones, protein, and oil in soybean seed.

Trait	Gene Name	Gene IDa	Role	Effectb	References
Isoflavones	F3H	Glyma.02g048400	Catalyze the biosynthesis of anthocyanin	−	Chen et al., 2021
	IFS	Glyma.07g202300	Encode of isoflavone synthase	+	Ku et al., 2020
	CHS7	Glyma.01g228700	Upstream gene	+	Dhaubhadel et al., 2007
	CHS8	Glyma.11g011500	Upstream gene	+	Dhaubhadel et al., 2007
	MYB11	Glyma.19g024700	Regulate element	+	Chen et al., 2021
Soy Protein	GY1	Glyma.03g163500	Catalyze the biosynthesis of glycinin	+	Nielsen et al., 1989
	MYB118	Glyma.10g048500	Regulate element	+	Zhang et al., 2009
	CG-1	Glyma.10g246300	Catalyze the biosynthesis of β-conglycinin	+	Kasuga et al., 1997
	PEPC	Glyma.12g229400	Encode phosphoenolpyruvate carboxylase	+/−	Hata et al., 1998
Soybean Oil	MYB73	Glyma.06g303100	Regulate element	+	Liu Y. F. et al., 2014
	FAD2-1A	Glyma.10g278000	Encode δ-12 fatty acid desaturase 2	+	Bolon et al., 2011
	DGK7	Glyma.13g093100	Encode diacylglycerol kinase 7	+	Arisz et al., 2009
	PEPC	Glyma.12g229400	Encode phosphoenolpyruvate carboxylase	+/−	Hata et al., 1998
Protein Plus Oil	PEPC	Glyma.12g229400	Encode phosphoenolpyruvate carboxylase	+/−	Hata et al., 1998
	GY1	Glyma.03g163500	Catalyze the biosynthesis of glycinin	+	Nielsen et al., 1989
	CG-1	Glyma.10g246300	Catalyze the biosynthesis of β-conglycinin	+	Kasuga et al., 1997
	FAD2-1A	Glyma.10g278000	Encode δ-12 fatty acid desaturase 2	+	Bolon et al., 2011

^aGene ID was based on Wm82.a2.v1 assemblies.

^bEffect: the symbol, “+,” refers to promote; the symbol, “−,” refers to restrain; the symbol, “+/−,” refers to regulate.