Skip to main content
. 2022 Jan 1;13(1):253–267. doi: 10.7150/jca.65687

Figure 2.

Figure 2

UCA1 functions as a miR-495 sponge. (A) UCA1 is primarily expressed in the cytoplasm. (B) Schematic comparison between UCA1 and the “seed sequence” of miR-495. (C) The miR-495 level was lower in not only Caco2-CR cells than in Caco2-CS cells but also in Caco2-UCA1 cells than in Caco2-NC cells. U6 served as an internal reference for normalization. (D) The luciferase activity of cells cotransfected with wild-type UCA1 and miR-495 mimics was significantly reduced compared with those cotransfected with wild-type UCA1 and miR-NC, while this repression of luciferase activity was completely refractory, after which cells were transfected with the UCA1 mutated vector. (E) Compared to the control immunoprecipitants, UCA1 and miR-495 were found to be preferentially enriched in Ago2-containing miRNPs. (F) Upregulated expression of miR-495 was found in Caco2-UCA1 cells transfected with miR-495 mimics compared with the mimic control group. U6 served as an internal reference for normalization. (G) miR-495 upregulation in Caco2-UCA1 cells decreased the IC50 of cetuximab. *P < 0.05, **P < 0.01.