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. 2022 Jan 1;13(1):62–75. doi: 10.7150/jca.66016

Figure 1.

Figure 1

Ago-RIP sequencing identifies new miR-449a-5p target genes. (A) Comparisons calculated to detect potential direct miR-449a-5p target genes and transcriptional changes considering the concept of linear contrasts. (B) Workflow of miR-449a-5p target gene filtering and definition of potential direct miR-449a-5p targets. C = Contrast, FC = fold change. (C) Validation of miR-449a-5p targets in HLE cells by quantitative Real-Time PCR. Relative expression of target RNAs was normalized to miR-control treated HLE cells. ****p<0.0001; ordinary one-way ANOVA with Dunnett's multiple comparisons test.