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. 2022 Jan 1;13(1):62–75. doi: 10.7150/jca.66016

Figure 4.

Figure 4

miR-449a and knockdown of PEA15, PPP1CA and TUFT1 exert distinct tumor suppressive functions. (A,B) HLE and Huh7 cells were transfected with 3 nM siRNA pools against the expression of either PEA15, PPP1CA or TUFT1. (A) Cell viability was analyzed by WST-1 assay and normalized to si-control (dotted line). (B) Apoptosis was analyzed by caspase 3/7 assay and normalized to cell viability and si-control (dotted line) (C) HLE and Huh7 cells were transfected with miR-449a-5p mimic or siRNA pools against the expression of either PEA15, PPP1CA or TUFT1. 48 h after transfection, protein expression of p-ERK 1/2, ERK 1/2, p-Akt and Akt was analyzed by western blotting with Actin and GAPDH as loading controls. Gels were processed in parallel. (D) Protein association network in STRING illustrating the interaction with MAPK1 (ERK) and AKT1. Line thickness indicates the strength of data support 25.