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. 2022 Jan 1;13(1):124–133. doi: 10.7150/jca.63578

Figure 3.

Figure 3

Proliferation of mouse colonic cells was increased after A. muciniphila administration. C57BL/6 mice were orally administrated with A. muciniphila or PBS and treated with AOM/DSS to induce CRC. The mice were sacrificed at day 80, and colon tissues were collected. Ki67 staining was applied using IHC method, and RT-qPCR and Western-blot were used to measure the expression of proliferation-related molecules. A. Ki67 staining results, scale bar: 100 um; B. The average numbers of Ki67+ cells in each crypt. C. Immunoblot analysis of PCNA in colon tissues; D. Colonic S100a9 gene expression; E, Snrpd1 gene expression. F, Dbf4 gene expression. (C-E) Data were represented by Mean ± SEM. PBS+AOM/DSS, n=5; AKK+AOM/DSS, n=7. *P<0.05, ns: no significantly different, B, D, E-F. Upaired student T test. The experiments were repeated twice independently.