Figure 2.

Immunogenicity of mRNA Immunogen-Specific Design Elements. (A, C) Serum samples 6 weeks post-boost were assessed for NiV pre-F specific IgG (A) or monomeric G-specific IgG (C) by ELISA. Line represents mean of all animals in each group +/- standard deviation. (B, D) VSVΔG-luciferase pseudovirus neutralization assays were performed on individual mouse sera collected 6 weeks post-boost. The log₁₀ reciprocal IC₈₀ neutralization titer for each sample was calculated by curve fitting and non-linear regression using GraphPad Prism. Line represents mean of log₁₀ reciprocal IC₈₀ dilution +/- standard deviation. P values were calculated using one-way ANOVA with Tukey’s multiple comparisons test (**p < 0.01). Dotted lines represent assay limits of detection.