Figure 3.

Immunogenicity of mRNA Pre-F/G Chimeric Design Elements. (A, B) Serum samples 6 weeks post-boost were assessed for NiV pre-F specific IgG (A) or monomeric G-specific IgG (B) ELISA. Line represents mean of all animals in each group +/- standard deviation. (C) VSVΔG-luciferase pseudovirus neutralization assays were performed on individual mouse sera collected 6 weeks post-boost. The log₁₀ reciprocal IC₈₀ neutralization titer for each sample was calculated by curve fitting and non-linear regression using GraphPad Prism. Line represents mean of log₁₀ reciprocal IC₈₀ dilution +/- standard deviation. P values were calculated using two-way ANOVA with Tukey’s multiple comparisons test (*p < 0.5, ***p < 0.001, ****p < 0.0001). Dotted lines represent assay limits of detection. All mRNA immunogens were codon-modified using Moderna’s proprietary codon algorithms designed to improve protein expression and mRNA manufacturability. In this figure, we specifically evaluated mRNA preparations that had used two different codon algorithms, referred to as “old” and “new”.