Figure 2.
Rapid induction and reversal of Gyki-induced PHP
(A) Left: Normalized median mEPSP amplitude, EPSP amplitude, and QC of a representative cell as a function of time before and after Gyki treatment. Values are normalized to respective mean baseline values before Gyki application. Right: Representative mEPSP and EPSP traces at specified time points. [Ca2+]e = 0.3 mM.
(B) Mean normalized mEPSP amplitude, EPSP amplitude, and QC as a function of time before and after Gyki treatment. The shaded region represents SEM around the mean. Values for individual cells are normalized to the respective mean baseline values before Gyki application. n = 15.
(C) Representative traces (mEPSPs and EPSPs), mEPSP amplitudes, EPSP amplitudes, and QC of NMJs after Ctrl or Gyki washout. n = 23 (Ctrl wash) versus 22 (Gyki wash). [Ca2+]e = 0.3 mM.
(D) Left: Normalized median mEPSP amplitude, EPSP amplitude, and QC of a representative cell as a function of time before and after Gyki wash. Values are normalized to respective mean baseline values before Gyki wash. Right: Representative mEPSP and EPSP traces at specified time points. [Ca2+]e = 0.3 mM.
(E) Mean normalized mEPSP amplitude, EPSP amplitude, and QC as a function of time before and after Gyki wash. The shaded region represents SEM. Values for individual cells are normalized to the respective mean baseline values before Gyki wash. n = 5.
(F) Representative EPSC traces from a Ctrl- and Gyki-treated NMJ measured at different [Ca2+]e.
(G) EPSC amplitude at different [Ca2+]e for Ctrl- and Gyki-treated NMJs. n = 16, 16, 51, 27 (Ctrl) versus 14, 14, 53, 23 (Gyki); Hill’s coefficient = 3.6 (Ctrl) versus 3.3 (Gyki). Error bars represent SEM.
(H) Inverse of squared coefficient of variation (CV−2) of EPSC amplitudes recorded at [Ca2+]e = 0.3 mM and estimated quantal size (q) for Ctrl- and Gyki-treated NMJs. n = 10 (Ctrl) versus 8 (Gyki).
(I) EPSC amplitude spread as a function of [Ca2+]e and relation between EPSC variance and mean for a sample variance-mean analysis, along with estimated q and functional release sites (N) for Ctrl- and Gyki-treated NMJs. n = 10 (Ctrl) versus 8 (Gyki). The error bars represent standard error of the estimator. The whiskers of the box plots extend to either 1.5 times the inter-quartile range or to the farthest data point, whichever is closest to the respective quartile. Individual data points are shown as gray dots. p, p value; r, effect size; ns: p ≥ 0.05; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.