REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Brp	DSHB	Cat# nc82, RRID:AB_2314866
Rabbit polyclonal anti-Discs large	Jan Pielage lab	NA
Mouse anti-GFP	Molecular Probes	Cat# mAb 3E6, RRID:AB_2313858
Goat Alexa-677 conjugated anti-HRP	Jackson ImmunoResearch	Cat# 123-605-021, RRID:AB_2338967
Alexa 488 conjugated anti-mouse	Molecular Probes	Cat# A-11029, RRID:AB_138404
Alexa-555 conjugated anti-rabbit	Molecular Probes	Cat# A-21429, RRID:AB_2535850
Atto594 conjugated anti-mouse	Sigma-Aldrich	Cat# 76085, RRID:AB_1137653
Chemicals, peptides, and recombinant proteins
Dulbecco’s Phosphate Buffered Saline	Sigma-Aldrich	D8537
Triton X-100	Sigma-Aldrich	X100
ProLong Gold Antifade	Thermo Fisher Scientific	P36930
ethanol	Merck	CAS# 64-17-5
KCl	Sigma-Aldrich	746436
NaCl	Sigma-Aldrich	71380
Na-HEPES	Sigma-Aldrich	H7006
HEPES	Sigma-Aldrich	H3375
Trehalose	Sigma-Aldrich	T9531
Sucrose	Sigma-Aldrich	84100
MgCl2	Fluka Analytical	63020
CaCl2	Fluka Analytical	21114
Philanthotoxin-433	Santa Cruz Biotechnology	Cat # sc-255421
γDGG	Tocris	Cat # 0112
Gyki-53655	Tocris	Cat # 2555
Experimental models: Organisms/strains
D. melanogaster: Control: w1118	Bloomington Drosophila Stock Center	BDSC: 3605; FlyBase: FBal0018186
D. melanogaster: D42-Gal4: w∗; P{GawB}D42	Bloomington Drosophila Stock Center	BDSC: 8816; FlyBase: FBti0002759
D. melanogaster: OK371-Gal4: w1118; P{GawB}OK371	Bloomington Drosophila Stock Center	BDSC: 26160; FlyBase: FBal0243489
D. melanogaster: MHC-Gal4: w1118; P{GawB}MHC	Graeme Davis lab	FlyBase: FBal0256907
D. melanogaster: Mef2-Gal4: w1118; P{GawB}Mef2	Monica Zwicky lab	FlyBase: FBal0288220
D. melanogaster: dysb1: w1118; PBac{RB}Dysbe01028/TM6B, Tb1	Dion Dickman lab	BDSC: 17918; FlyBase: FBal0161105
D. melanogaster: rimΔ103: w1118; rimΔ103/TM6B, Tb1	Graeme Davis lab	FlyBase: FBal0161105
D. melanogaster: GluRIIASP16: w∗; GluRIIASP16/CyO	Graeme Davis lab	FlyBase: FBal0085982
D. melanogaster: GluRIIBSP5: w∗; GluRIIBSP5	Dion Dickman lab	NA; Muttathukunnel et al., 2021
D. melanogaster: KaiR1D2: y1w67c23; Mi{ET1}KaiR1DMB01010	Bloomington Drosophila Stock Center	BDSC: 22962; FlyBase: FBst0022962
D. melanogaster: ppk11Mi: w1118; Mi{ET1}ppk11MB02012	Bloomington Drosophila Stock Center	BDSC: 23781; FlyBase: FBst0023781
D. melanogaster: dmpΔC: w∗; dmpΔC	Graeme Davis lab	NA; Wang et al., 2014
D. melanogaster: inckk3: w1118; inckk3	Dion Dickman lab	NA; Kikuma et al., 2019
D. melanogaster: PKDH: y1w∗; Mi{MIC}PKDMI03619	Bloomington Drosophila Stock Center	BDSC: 37604; FlyBase: FBal0265471
D. melanogaster: PKDGFP: y1w∗; Mi{PT-GFSTF.0}PKDMI09308-GFSTF.0/TM3, Sb1 Ser1	Bloomington Drosophila Stock Center	BDSC: 60273; FlyBase: FBst0060273
D. melanogaster: RNAi of PKD: y1v1; P{TRiP.HMC04179}attP2/TM3, Sb1	Bloomington Drosophila Stock Center	BDSC: 55898; FlyBase: FBst0055898
D. melanogaster: UAS-PKDWT: y1w67c23; P{UAS-PKD.GFP}	Anette Preiss lab	FlyBase: FBal0212370
Software and algorithms
Fiji / ImageJ	https://fiji.sc	RRID: SCR_002285
Clampex	Axon CNS, Molecular Devices	RRID: SCR_011323
Leica Application Suite X	Leica Microsystems	RRID:SCR_013673
NumPy	https://www.numpy.org/	RRID:SCR_008633
SciPy	https://www.scipy.org/	RRID:SCR_008058
IPython	http://ipython.org	RRID:SCR_001658
Neo	http://neuralensemble.org/neo/	RRID:SCR_000634
MatPlotLib	https://matplotlib.org/	RRID:SCR_008624
scikit posthocs	https://github.com/maximtrp/scikit-posthocs	RRID:SCR_021363
Biorender	http://biorender.com	RRID:SCR_018361