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. 2021 Dec 8;11:734959. doi: 10.3389/fonc.2021.734959

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Copyright © 2021 Pullikuth, Routh, Zimmerman, Chifman, Chou, Soike, Jin, Su, Song, Black, Print, Bedognetti, Howard-McNatt, O’Neill, Thomas, Langefeld, Sigalov, Lu and Miller

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of TREM1 expression in the breast tumor microenvironment by IF and single-cell RNAseq. (A) Representative patterns (P) of TREM-1 (red) and CD68 (green) immunofluorescent staining in breast tumor sections. DRAQ5-stained nuclei are shown in blue pseudocolor. (B) Single-cell RNA sequencing was performed on freshly dissociated cells of a stage III primary triple negative breast tumor. Expression profiles of 770 cells were K-means clustered and resolved spatially in a tSNE plot. (C) Cell identities in (B) were assigned based on significant expression of canonical marker genes. Mean-centered averages of cluster-specific gene expression are shown in the heatmap. (D) Relative expression levels of TREM1, CD68, MRC1 (M2 macrophage marker) and OLR1 (MDSC marker) are shown.