FIG. 3.

Dose-dependent activation of SREBP-1c promoter by LXRs (left panel) and their ligands (right panel). (Left panel) pBP1c2600-Luc was cotransfected into HEK 293 cells with a positive clone expressing LXR from the screening (pCMV-LXRα or pCMV-LXRβ), an empty vector (CMV-7) used as a control, or pSV-βgal, which was used as a reference plasmid. (Right panel) Ligands for LXR or ethanol (used as a control) were added to the cells after transfection of pBP1c2600-Luc and pSV-βgal in medium with 10% fetal bovine serum 24 h prior to the assay. After the incubation, luciferase activity was measured and normalized to β-galactosidase activity. The fold induction of luciferase activity by LXRs or their ligands (means ± standard deviations in the left panel [n = 3]; means in the right panel [n = 2]) compared to that of controls is shown. Cho, cholesterol.