FIG. 8.

Synergistic activation of the LXRE complex in the SREBP-1c promoter by LXRs, RXR, and their ligands. The luciferase reporter construct containing the LXRE complex in the mouse SREBP-1c promoter (pLXRE-Luc) was cotransfected into HEK 293 cells with pCMV-LXRα or -β, pRXR, or both and pSV-βgal. After the transfection, the cells were incubated in the absence or presence of the ligand 22(R)-HC, 9CRA, or both for 24 h. Luciferase activity was measured and normalized to β-galactosidase activity. Fold induction (means ± standard deviations) compared to that of the control (an empty expression vector with no addition of ligands) is shown.