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. 2021 Dec 8;9:792111. doi: 10.3389/fbioe.2021.792111

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Copyright © 2021 Wei, Wang, Xiang, Jiang, Gong, Su, Al Azad, Dong, Feng, Wu, Chan, Yang and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characteristics of vNAR sequences from immunized library. (A) Schematic for vNAR phage library construction from immunized sharks. (B) Amino acid variability for vNAR sequences (N = 6,000). (C, D) Sequence alignment (C) and percentages (D) of different vNAR types in the library. (E) Percentages of vNARs with different cysteine numbers.